Acute aquatic toxicity tests with Daphnia Magna have also been conducted. Table salt (NaCl), diesel, and B100 were compared to each other. The LC50 count (the concentration where 50 percent of the Daphnia Magna have died and 50 percent were still alive) for table salt was 3.7 parts per million (ppm). Fifty percent of the Daphnia Magna were dead at 1.43 ppm for diesel fuel. The LC50 number varied for B100 from 23 ppm to 332 ppm. Therefore, B100 is less toxic than diesel fuel.
The 96-hr LC50 (Lethal Concentration) for Bluegills for B100 was greater than 1,000 mg/L. Concentrations above 1,000 mg/L are deemed "insignificant" according to US National Institute for Occupational Safety and Health Guidelines in its Registry of the Toxic Effects of Chemical Substances.
B100 is considered biodegradable based on its chemical nature and test data collected for experimentally determined oxygen demand and carbon dioxide production as a percent of calculated theoretical values. B100 does not show any micro biological inhibition up to 10,000 mg/L.
In tests performed by the University of Idaho, B100 in an aqueous solution after 28 days was 95% degraded. Diesel fuel was only 40% degraded. In a second study done in an aquatic environment (CO2 evolution), various B100 products were 85.5% to 88.5% degraded in 28 days, which is the same rate as sugar (dextrose). Diesel degradation was only 26.24%.
B100 helps speed diesel degradation when used in blends with petroleum diesel fuel.
B100 degrades about four times faster than petroleum diesel fuel. Also, when blended with B100, the degradation rate of petroleum diesel tripled when compared to diesel alone, according to a University of Idaho tests.